We will study the daily to seasonal and spatial variability in distribution, abundance and biomass of phytoplankton, zooplankton, and sinking particles and construct size spectra. A unique ensemble of in-situ optical and acoustic instrumentation will be deployed to investigate diel vertical migration of zooplankton and nekton, i.e., Underwater Vision Profiler (UVP) and zooplankton hydroacoustic profiler. Multifactorial laboratory and cruise experiments will be used to assess the interactive effects of different climate change-related stressors upon phytoplankton productivity.